RNA-seq Analysis Montreal and St. John's 2026 | 02: RNA-seq alignment concepts and file formats
Bioinformatics DotCa
This module explores the fundamental strategies for RNA-seq analysis, distinguishing between mapping, pseudo-alignment, and de novo assembly as primary approaches to handling sequencing reads. Mapping utilizes tools like STAR or HISAT2 to align reads base-by-base against a reference genome, offering …