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Martian Regolith as a Substrate for Foreign Planetary Horticulture

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Rachel Tucker, an undergraduate student at the University of Florida, presented research on using Martian regolith as a substrate for growing crops in space exploration contexts. As human missions become longer and travel further from Earth, utilizing local resources becomes essential to maximize available supplies without relying entirely on transport from home. To study this potential, researchers utilized Mars Global Simulant 1, a specific mixture designed by Exolith Labs to accurately mimic the mineral, chemical, volatile, and spectral properties of regolith found in Gale Crater, which is currently the best-characterized Martian soil sample available for testing. The experiment aimed to determine how low concentrations of this simulant affect crop growth compared to an inert perlite base. Researchers tested three specific mixtures containing 10%, 25%, and 50% Mars Global Simulant alongside a control group with no regolith, planting twenty-five seeds each for six different cultivars including daikon radish, mustard greens, bok choy, buckwheat, kale, and red romaine lettuce. Daily data collection tracked seedling emergence stages such as root appearance, cotyledon development, and the first true leaf budding, while final harvest measurements included fresh weight, dry weight, plant height, and stem diameter to assess overall vegetative health across different growth periods. The results clearly indicated that increasing concentrations of the simulant led to a significant decrease in vegetative tissue for all tested plants. Statistical analysis revealed major differences between the 10% and 25% concentration groups regarding seedling emergence rates, with even more severe impacts observed at the 50% level where there was insufficient plant growth to collect meaningful harvest data. While some cultivars like red romaine showed slightly different responses, five out of six plants exhibited statistically significant reductions in fresh weight, dry weight, height, and stem diameter when grown in regolith mixtures compared to the control group, demonstrating that even small amounts of unaltered simulant severely inhibit crop development. These findings conclude that raw Martian regolith cannot be used directly for agriculture without modification because it actively suppresses plant growth at concentrations far lower than previously thought possible. The study emphasizes that before such soil can become a viable resource for sustaining life on Mars, scientists must develop methods to alter its chemical or physical properties to remove these inhibitory effects. This research underscores the collaborative nature of space science and highlights the critical need for further experimentation to ensure future colonists can successfully grow their own food using local Martian resources rather than relying solely on supplies brought from Earth.
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Hello. Thank you for checking out my lightning talk. I'm Rachel Tucker, an undergraduate student at the University of Florida, and I've been studying Martian regolith as a substrate for foreign planetary horticulture. Why do regolith studies matter? Human space exploration is increasing in duration and distance, which poses a greater need for in situ resource utilization in order to maximize available resources. A regolith stimulant offers a comparable substrate in order to study the potential of Martian regolith as a local resource for crop production on Mars. Why do we want to look at Mars Global Simulant 1? Other Martian simulants are available, such as JSC Mars 1 and Mojave Mars Simulant. Both are sourced from natural environments and have been widely studied in literature. Mars Global Simulant 1 is a recipe created by Exolith Labs to mimic the mineral, chemical, volatile, and spectral properties of the rockness regolith at Gale Crater, which is the best characterized regolith to date. In our experiment, we wanted to determine what low concentrations of the Mars Global Simulant, how that could affect crops. And so, we tested three different concentrations of regolith relative to an inert arcelite substrate base. We tested 10%, 25, and 50% Mars Global Simulant 1 relative to the arcelite. During growth, we we collected daily collected data daily for radical emergence, cotyledon emergence, first true leaf budding, and at 10 days after planting, we harvested and collected fresh weight, dry weight, height, and widest diameter. We We planted 25 seeds for each of the following cultivars: daikon radish, amara mustard, extra dwarf bok choy, buckwheat, red Russian kale, and outredgeous red romaine. Pictured here is a top view of 3, 5, 7, and 10 days after planting of the control group in the top row, 10%, 25%, and the 50% concentration of Mars Global Simulant. As you can see, as the concentration of Mars Global Simulant increases, the amount of vegetative tissue decreases. Pictured here is a graph for each of the cultivars. In the x-axis is the number of days after planting, and in the y-axis is the percent of seeds planted. As you can see here for radical emergence, there's a large difference in each of the six cultivars between the 25% concentration of regolith and the 50% concentration. For cotyledon emergence, in five of the six cultivars, with the exception of outredgeous red romaine, there was a large difference between the 10% and the 25% concentration of regolith. And again, for the first true leaf budding, for five of the six cultivars, with the exception of the outredgeous red romaine, there was a large difference between the 25% and the 10% concentrations of the regolith. At harvest, there was not enough vegetative tissue in order to collect data for the 50% concentration of regolith. However, for the 20 fresh weight for the 25% concentration of regolith relative to the control group, each of the six cultivars was statistically significant in fresh weight. For dry weight, five of the six cultivars were statistically significant between the 25% concentration of regolith and the test and the control group. For the height for the harvest data, it was very statistically significant in terms of the 25% concentration of regolith, in which all six cultivars were statistically significant. And in 10% concentration of regolith, five of the six cultivars were statistically significant. And as you can see pictured here, the extra dwarf bok choy at 0% or the control group and the 10% concentration, there is a wide difference. Diameter was also greatly affected. In for all six cultivars, the 25% concentration of regolith was statistically significant from the control group. What does this mean? Unamended Mars Global Simulant 1 severely inhibits vegetative crop growth in multiple cultivars, and not just at 100% concentration. This occurs at low concentrations as well. And what this means is that this this regolith will need to be altered in some way in order to become a viable resource for crop growth on Mars. Of course, this is not done as one person. Science takes a lot of people, and a lot of wonderful people contributed to this product project. Thank you so much for listening. I hope you enjoyed the project.